The Testosterone, Free, Direct with Total Testosterone test measures both the free (unbound) and total testosterone levels in the blood using liquid chromatography-tandem mass spectrometry (LC/MS-MS). This comprehensive assessment is valuable for evaluating androgen status in patients with symptoms of hormonal imbalance, such as infertility, sexual dysfunction, or irregular menstrual cycles. It helps differentiate between primary and secondary hypogonadism and is also useful for monitoring testosterone replacement therapy.