Lab Education
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May 17, 2024

GI-MAP Result Interpretation 101: A Guide for Healthcare Practitioners

Written By
Brittney Bertagna RN
Medically Reviewed by
Updated On
January 14, 2025

Maintaining a healthy gut is important for overall well-being, as it can influence various aspects of health. In this guide, we explore GI-MAP testing—a tool that can help identify unique characteristics of a person's gut microbiome.

Whether you're a healthcare practitioner considering GI-MAP testing for your practice or simply looking to learn more about it, this guide will help you understand this diagnostic tool.

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What is the GI-MAP Test?

The GI-MAP, or Gastrointestinal Microbial Assay Plus, test uses quantitative polymerase chain reaction (qPCR) technology to assess the microbial composition of the gastrointestinal tract.

A GI-MAP test can detect various microorganisms that may be associated with gastrointestinal issues. By analyzing a stool sample, the test can provide information about different microorganisms and offers several benefits for understanding gastrointestinal health.

In addition, the test assesses the abundance and diversity of commensal bacteria, which can help identify imbalances in the gut microbiome. An imbalanced gut microbiome is sometimes associated with conditions like irritable bowel syndrome.

Furthermore, the GI-MAP test identifies opportunistic pathogens, which are usually harmless but can contribute to gastrointestinal symptoms under certain circumstances. This information provides insights into potential sources of gastrointestinal issues.

Why Order a GI-MAP Test?

Ordering a GI-MAP test can be beneficial for healthcare professionals because of its potential to provide detailed insights into the gut microbiome. This analysis can help identify microorganisms that might otherwise go unnoticed, leading to a more comprehensive understanding of gut health.

The GI-MAP test is a component of functional medicine that allows for personalized approaches based on the microbiome profile of each individual. Since each person's gut microbiome is unique, understanding these differences can help in managing digestive health.

The information gained from this test can assist in developing personalized wellness plans, considering the individual's microbiome characteristics. For example, if the test suggests an overgrowth of certain bacteria, healthcare providers might consider strategies to address this.

If commensal bacteria are found to be imbalanced, recommendations for probiotic supplementation or dietary changes might be suggested to support a healthier microbiome.

When to Consider a GI-MAP Test for Your Patients

One reason to consider testing is when patients have ongoing digestive complaints, such as abdominal discomfort, bloating, diarrhea, or constipation, and other tests have not provided clear answers. A GI-MAP test can help identify potential microbial imbalances that may be contributing to these symptoms.

Another scenario is when gastrointestinal infections are suspected, such as those from food or parasites. The GI-MAP test can provide information about the presence of microorganisms that might be involved, aiding in understanding the situation better.

Additionally, patients with chronic conditions, such as diabetes, obesity, or autoimmune disorders, might benefit from regular assessments of their gut health. The GI-MAP test can offer insights into the microbial composition and identify any imbalances that may be relevant to their health management.

Ordering a GI-MAP test can help assess the microbial composition, identify patterns, and guide strategies to support health outcomes in patients with autoimmune conditions.

In addition to initial insights, the GI-MAP test can be useful for follow-up assessments. By retesting after interventions, healthcare professionals can evaluate the effectiveness of strategies and make informed decisions about any necessary adjustments.

This could involve checking for changes in specific microorganisms after targeted interventions or assessing shifts in beneficial bacteria or overall microbial diversity following changes such as probiotic use or dietary adjustments.

How to Order a GI-MAP Test Through Rupa Health

It only takes a few minutes to order specialty lab tests through Rupa Health.

Set up an Account: Visit the Rupa Health website to create an account.

Log In: Once your account is created, log in to the Rupa Health platform using your credentials.

Navigate to the Lab Test Catalog: Within the platform, locate the Lab Test Catalog. There you’ll find a catalog of all tests offered through Rupa Health.

Search for the GI-MAP Test: Use the search function or browse through the available tests to find the GI-MAP test. Click on the test to access more details.

Add the GI-MAP Test to Cart: Click the "Add to Cart" to include it in your order.

Provide Patient Information: Fill in the required patient information for whom the GI-MAP test is being ordered. Ensure that the information is accurate and complete.

Review Order Summary: Before proceeding to checkout, review the order summary to ensure that the correct test, or tests, and patient information are selected.

Interpreting GI-MAP Results

The GI-MAP results report provides a comprehensive analysis of various components related to gastrointestinal health.

Understanding the Results Report

The microbial flora analysis section provides information about the presence and quantity of bacterial, parasitic, and viral microorganisms that are known to be associated with intestinal issues. The report will indicate whether any microorganisms were detected and provide their respective quantities.

Specific bacterial microorganisms that were identified are listed in the bacterial section. Examples include Campylobacter, C. difficile Toxin A and B, Enterohemorrhagic E. coli, Salmonella, and more. These microorganisms have the potential to be associated with a range of gastrointestinal symptoms.

The parasitic section focuses on the detection of parasites such as Cryptosporidium, Entamoeba histolytica, and Giardia. These parasites can be linked to gastrointestinal issues and may require specific attention.

The report also identifies viral microorganisms found in the stool, such as Adenovirus and Norovirus. These viruses can be associated with gastrointestinal symptoms.

The presence or absence of Helicobacter pylori (H. pylori), a bacterium associated with ulcers and gastrointestinal symptoms, is indicated in a specific section. Additionally, the report may analyze the genetic potential of a specific H. pylori strain to cause issues.

Assessing the levels of beneficial bacteria in the gut is an important aspect covered in the results. Beneficial bacteria play a role in maintaining gut health, nutrient absorption, immune function, and protection against potential pathogens.

The bacterial phyla ratio section reports the ratio between two major groups of bacteria in the gut, Bacteroidetes and Firmicutes. An abnormal ratio may indicate an imbalance in the microbiome, which has been associated with various health concerns.

The report also identifies opportunistic bacteria that are typically harmless in small amounts but can cause problems if overgrown, especially in individuals with compromised immune systems. These bacteria may be linked to symptoms like diarrhea, abdominal pain, and constipation.

Detection of fungal organisms and yeast, including Candida species, is covered in the fungi/yeast section. Overgrowth of these organisms can lead to problems, particularly in individuals with compromised gut health.

Lastly, the report identifies both pathogenic and non-pathogenic parasites, such as protozoa (e.g., Blastocystis hominis) and worms (e.g., Ascaris lumbricoides). The detection of these parasites may indicate the need for specific attention.

Clinical Correlations

Healthcare practitioners can gain a more comprehensive understanding of a patient's gut health by integrating GI-MAP results with the patient's presenting symptoms and additional diagnostic tests. Identifying patterns or associations between specific microbial imbalances, microorganism presence, or dysbiosis markers and the patient's symptoms provides valuable insights.

For example, if a patient reports chronic diarrhea and the GI-MAP report reveals high levels of Clostridium difficile, it suggests a potential issue contributing to their symptoms.

If the patient has elevated inflammatory markers and the GI-MAP report indicates increased levels of certain bacteria, it may suggest an inflammatory condition or an issue requiring attention.

Additionally, assessing the patient's response to previous or ongoing interventions is another important aspect.

Monitoring whether there has been improvement or resolution of symptoms after targeted interventions for specific microorganisms identified in the GI-MAP report can help validate the relevance of the findings and guide further decisions.

It is also essential to consider the patient's individual context, including their medical history, current medications, dietary habits, and lifestyle factors. These factors can influence the gut microbiome and impact the interpretation of GI-MAP results.

For example, certain medications, such as proton pump inhibitors, can alter the gut microbiota composition, leading to potential imbalances that may be reflected in the GI-MAP report.

Read Some Real Case Examples:

Integrating GI-MAP Results into Patient Care

The results of the GI-MAP test provide helpful information that can inform decisions regarding dietary considerations, probiotics, antimicrobials, or other targeted approaches.

Treatment Implications

One significant implication of GI-MAP results is the identification of potential food sensitivities or intolerances. By analyzing the levels of antibodies against specific components like gliadin, which is found in gluten, the test can suggest a gluten sensitivity.

In such cases, healthcare providers may suggest a gluten-free diet to help manage symptoms and support gut health. Similarly, if the results reveal imbalances in certain bacteria, dietary modifications such as reducing sugar intake or increasing fiber consumption may be considered to support a healthier gut microbiome.

The GI-MAP results also play a role in guiding the selection of probiotics for microbiome support. The choice of probiotics can be tailored based on the specific strains identified in the GI-MAP results, ensuring a targeted approach to gut health.

In cases where the GI-MAP identifies certain bacteria, parasites, or fungal overgrowth, targeted interventions may be necessary. The test results help in selecting appropriate strategies that specifically address the identified microorganisms.

Furthermore, the GI-MAP results may suggest the need for other targeted approaches. For instance, if the test indicates high levels of markers associated with inflammation, healthcare providers may consider interventions to support immune health.

Additionally, if specific markers related to digestion or absorption issues are identified, targeted interventions such as digestive enzyme support or therapies to support pancreatic function may be considered.

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Key Takeaways

  • The GI-MAP test is a tool that can help identify microorganisms associated with gastrointestinal issues and assess the abundance and diversity of commensal bacteria to identify imbalances.
  • Ordering a GI-MAP test can benefit healthcare professionals by providing insights into the gut microbiome and helping to tailor wellness plans accordingly.
  • The test can be ordered through the Rupa Health platform, which provides a convenient and streamlined process for healthcare practitioners.
The information in this article is designed for educational purposes only and is not intended to be a substitute for informed medical advice or care. This information should not be used to diagnose or treat any health problems or illnesses without consulting a doctor. Consult with a health care practitioner before relying on any information in this article or on this website.

Learn more

Lab Tests in This Article

Cloyd, Jaime. “5 Lab Tests You Need to Know about If You Want to Test for Gluten Sensitivity.” Rupa Health, 21 Mar. 2023, www.rupahealth.com/post/5-lab-tests-you-need-to-know-about-if-you-want-to-test-for-gluten-sensitivity. Accessed 26 Apr. 2024.

“Understanding the Gut Bacteria Clostridium: What Does High and Low Levels Mean: How to Test and Treat.” Rupa Health, 23 May 2023, www.rupahealth.com/post/clostridium-101-what-does-high-and-low-levels-mean-how-to-test-increase-and-treat.

Davey, Julie Ann. “Julianna’s Battle with IBS, Weight Gain, and the Common Pathogen Helicobacter Pylori: A Case Study.” Rupa Health, 1 Aug. 2023, www.rupahealth.com/post/juliannas-battle-with-ibs-weight-gain-and-the-common-pathogen-helicobacter-pylori-a-case-study. Accessed 26 Apr. 2024.

DeCesaris, Laura. “What Is Gut Dysbiosis? 7 Signs to Watch For.” Rupa Health, 6 June 2022, www.rupahealth.com/post/how-your-gut-bacteria-affects-your-overall-health.

“GI-MAP by Diagnostic Solutions.” Rupa Health, www.rupahealth.com/lab-tests/diagnostic-solutions-gi-map.

Greenan, Shawn. “A Functional Medicine Approach to IBS.” Rupa Health, 5 Nov. 2021, www.rupahealth.com/post/a-functional-medicine-approach-to-ibs.

Henry, Eve. “Stool Testing 101: A Complete Guide to the Top 4 Gut Health Tests.” Www.rupahealth.com, www.rupahealth.com/post/stool-testing-101-a-complete-guide-to-the-top-4-microbiome-tests.

“Https://Www.rupahealth.com/Labs/Comprehensive-Guide-To-The-Gi-Map-Test.” Rupa Health, www.rupahealth.com/labs/comprehensive-guide-to-the-gi-map-test. Accessed 26 Apr. 2024.

Missimer, Arianna. “A Case Study of Unlocking the Gut-Brain Connection in Mast Cell Activation and Dysautonomia.” Rupa Health, 19 Oct. 2023, www.rupahealth.com/post/unlocking-the-gut-brain-connection-in-mast-cell-activation-and-dysautonomia. Accessed 26 Apr. 2024.

Teeter, Lauren Ann. “4 Lab Test That Can Help You Explore the Connection between Inflammation and Your Patients Metabolic Health.” Rupa Health, 18 May 2023, www.rupahealth.com/post/the-connection-between-inflammation-metabolic-health.

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